an efficient method for dna extraction from paraffin wax embedded tissues for pcr amplification of human and viral dna
Authors
abstract
background and objective: formalin-fixed paraffin-embedded tissues are a valuable source of dna for molecular studies. we designed and optimized an efficient procedure for dna extraction from formalin-fixed paraffin embedded tissues. materials and methods: seventy three blocks of cervical paraffin-embedded tissues were investigated. dna was extracted using 45 minutes boiling in alkaline solution together with 10 beads of chelex-20, followed by phenol-chloroform extraction and alcohol precipitation. results: this method produced dna suitable for amplification using primers specific for human smn and β globin genes in 98.63% and 82.2% of samples respectively. we also detected human papillomavirus dna in 58.33% of appropriate samples. conclusion: this procedure provides simple and efficient method for recovery of amplifiable genomic and viral dna from paraffin wax embedded tissues.
similar resources
An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA
Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...
full textEvaluation of extraction methods from paraffin wax embedded tissues for PCR amplification of human and viral DNA.
AIM To evaluate the efficiency of phenol/chloroform, microwave, and Qiagen spin column based DNA extractions from paraffin wax embedded tissue for use in the polymerase chain reaction (PCR). In addition, to assess the reliability of amplifying a housekeeping gene to indicate successful viral DNA extraction. METHODS DNA samples extracted from 20 blocks of cervical carcinoma tissues using the t...
full textAn efficient protocol for genomic DNA extraction from formalin-fixed paraffin-embedded tissues.
Formalin-fixed paraffin-embedded tissues (FFPET) represent the largest source of archival biological material available for genomic studies. In this work we present an advanced protocol for extraction of high quality DNA from FFPET that can be applied in several molecular studies. Although cat mammary tumours (CMT) are the third most frequent tumour in cats the recovery of significant number of...
full textThe Comparison of different Procedures for DNA extraction from paraffin-embedded Tissues: A commercial kit and a traditional method based on heating
Abstract Background and objectives: Paraffin-embedded tissues and clinical samples are a valuable resource for molecular genetic studies, but the extraction of high-quality genomic DNA from this tissues is still a problematic issue. In the Present study, the efficiency of two DNA extraction protocols, a commercial kit and a traditional method based on heating and K Proteinase was compared. Mate...
full textAn alternative protocol for DNA extraction from formalin fixed and paraffin wax embedded tissue.
BACKGROUND DNA extraction from paraffin wax embedded tissue requires special protocols, and most described methods report an amplification success rate of 60-80%. AIMS To propose a simple and inexpensive protocol consisting of xylene/ethanol dewaxing, followed by a kit based extraction. METHOD Xylene/ethanol dewaxing was followed by a long rehydration step and a kit based DNA extraction ste...
full textAn optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experiments
In diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. If not inactivated properly, cellular and/or environmental DNA nucleases will degrade genomic material during the extraction stage, and therefore might give rise to incorrect results in PCR experiments. When crude cell extracts, proteinase K–treat...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of pathologyPublisher: iranian society of pathology
ISSN 1735-5303
volume 3
issue 4 2008
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023